Production of a mitochondrial-DNA identical cloned foal using oocytes recovered from immature follicles of selected mares.
In ‘52nd Annual Convention of the American Association of Equine Practitioners Proceedings’, Vol. N., Jordan, R., Luzzani, C., Miriuka, S., Radrizzani, M., Donadeu, F. In vitro and in vivo development of horse cloned embryos generated with i PSCs, mesenchymal stromal cells and fetal or adult fibroblasts as nuclear donors. | Ortiz-Escribano, N., Bogado Pascottini, O., Woelders, H., Vandenberghe, L., De Schauwer, C., Govaere, J., Van den Abbeel, E., Vullers, T., Ververs, C., Roels, K., Van De Velde, M., Van Soom, A., and Smits, K. An improved vitrification protocol for equine immature oocytes, resulting in a first live foal. Effect of the number of passages of fetal and adult fibroblasts on nuclear remodelling and first embryonic division in reconstructed horse oocytes after nuclear transfer. | Miragaya, M., Revora, M., Rigali, F., Herrera, C., Viviani, L., Quintans, C., Pascualini, S., and Losinno, L. First equine clone born in Argentina by somatic cell nuclear transfer from a polo Argentino mare. Compounds used to produce cloned animals are genotoxic and mutagenic in mammalian assays in vitro and in vivo. Equine cloning can be performed using several different approaches, each with different rates of success. In this review we cover the history and applications of equine cloning and summarise the major scientific advances in the development of this technology in horses. Interspecific and extraspecific equine pregnancies. | Lagutina, I., Lazzari, G., Duchi, R., Colleoni, S., Ponderato, N., Turini, P., Crotti, G., and Galli, C. Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type. Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system. Artificial activation of bovine and equine oocytes with cycloheximide, roscovitine, strontium, or 6-dimethylaminopurine in low or high calcium concentrations. | Galli, C., Colleoni, S., Duchi, R., Lagutina, I., and Lazzari, G. Developmental competence of equine oocytes and embryos obtained by in vitro procedures ranging from in vitro maturation and ICSI to embryo culture, cryopreservation and somatic cell nuclear transfer. The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos. | Gambini, A., Jarazo, J., Karlanian, F., De Stéfano, A., and Salamone, D.